Luo D, Liu WX, Wang YR, Zhang JY, Liu ZP*.Development of a rapid one-step PCR protocol to distinguish between alfalfa (Medicago sativa) and sweet clover (Melilotus spp.) seeds.Seed Science and Technology.2014, 42: 237-246.

影响因子: 0.70

摘要:Distinguishing seeds of alfalfa (Medicago sativa) and sweet cover (Melilotus spp.) from one another is difficultbecause of similarities in the colour, shape, size and weight of the seeds. Existing methods to distinguishthese seeds are usually time-consuming and inaccurate. In our study, a polymerase chain reaction (PCR) andpolyacrylamide gel electrophoresis (PAGE) method was developed to distinguish alfalfa and sweet clover seedsfrom one another. Based on differences in the DNA sequences of plastid matK spacer regions, a pair of primerswas designed and used with simple PCR and PAGE techniques to identify 45 alfalfa and 34 sweet clover (8white and 26 yellow) accessions that were collected from all over the world. Our results with this pair ofprimers were clearly different between the two species, supporting the reliability, sensitivity and simplicity ofthis method.